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SERIAL DILUTIONS – TUBE METHOD Principle Serial dilution is a common technique used in many immunologic procedures. A small amount of serum or solute can be serially diluted by transferring aliquots to diluent.

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• Serial Dilution Microbiology Lab
• Serial Dilutions Microbiology Formula
• Serial Dilution Test Microbiology

Serial Dilution Microbiology Lab

1. Serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. From this, a calculation of viable cells in the original suspension can be made, as a colony picked for pure culture.
2. Sep 09, 2014  This general microbiology practical lecture explains the serial dilution techniques in pour plate method to isolate bacteria. For more information, log on to.
3. This general microbiology practical lecture explains the serial dilution techniques in pour plate method to isolate bacteria. For more information, log on to.

A serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M .. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10^0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10^0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.

In biology and medicine[edit]

In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results.^[1] Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators.^[2]

In homeopathy[edit]

Serial Dilutions Microbiology Formula

Serial dilution is one of the core foundational practices of homeopathy, with 'succussion', or shaking, occurring between each dilution. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain.^[3][4]

Paradise, since neither are able to save Bevel.While Bevel's drowning in the river that promised him baptism and eternal life, that promised him that he would 'count' for something, is a grotesquely humorous irony, typical of O'Connor's stories, it might be pointed out that Bevel does indeed seem to experience an 'epiphany' of sorts as he is swept away to his death; 'for an instant he was overcome with surprise; then, since he was moving quickly and knew that he was getting somewhere, all his fury and his fear left him.' Baptism in christian theology has long been associated with death and christian detachment; in baptism a christian enters into the death of Christ, undergoing a 'dying to sin' and a 'dying to self.' It is perhaps this dying to self that Bevel is experiencing as 'his fury and fear leave him' and why 'he knew he was getting somewhere.'

See also[edit]

References[edit]

Serial Dilution Test Microbiology

1. ^K. R. Aneja. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. 69. ISBN81-224-1494-X
2. ^Booth, C.; et al. (2006). Extremophiles. Methods in microbiology 35. Academic Press. p. 543. ISBN978-0-12-521536-7.
3. ^Weissmann, Gerald (2006). 'Homeopathy: Holmes, Hogwarts, and the Prince of Wales'. The FASEB Journal. 20 (11): 1755–1758. doi:10.1096/fj.06-0901ufm. PMID16940145. Retrieved 2008-02-01.
4. ^Ernst, Edzard (November 2005). 'Is homeopathy a clinically valuable approach?'. Trends in Pharmacological Sciences. 26 (11): 547–548. CiteSeerX10.1.1.385.5505. doi:10.1016/j.tips.2005.09.003. PMID16165225.

• Michael L. Bishop, Edward P. Fody, Larry E. Schoeff. Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24. ISBN0-7817-4611-6.

External links[edit]

• How to Make Simple Solutions and Dilutions, Bates College